Selective Enzymatic Determination of L-Phenylalanine and Phenyl-Pyruvate
作者:
Toshihisa Ohshima,
Hiroko Sugimoto,
Kenji Soda,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 12
页码: 2205-2215
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808059903
出版商: Taylor & Francis Group
关键词: phenylalanine dehydrogenase;enzymatic determination;determination of phenylalanine and phenylpyruvate
数据来源: Taylor
摘要:
A simple enzymatic end point method for the determination of L-phenylalanine and 3-phenylpyruvate has been developed. The assay is based on the spectrophotometry determination of NADH formed or degraded in the reaction catalyzed with Thermoactinomyces intermedins phenylalanine dehydrogenase. This method enables the simple and selective determination of L-phenylalanine and 3-phenylpyruvate based on the high substrate specificity of the enzyme. The assay is sensitive in the range of 0.015−0.15 umol of L-phenylalanine and 3-phenylpyruvate.
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