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A simple chamber for observing microscopic specimens in both top and side views

 

作者: C. A. Boocock,   A. F. Brown,   G. A. Dunn,  

 

期刊: Journal of Microscopy  （WILEY Available online 1985）
卷期: Volume 137, issue 1  

页码: 29-34

 

ISSN:0022-2720

 

年代: 1985

 

DOI:10.1111/j.1365-2818.1985.tb02558.x

 

出版商: Blackwell Publishing Ltd

 

关键词: Optical systems;side view microscopy;light microscopy;Nomarski (DIC);phase contrast;fluorescence microscopy;three‐dimensional substrata;collagen gels;tissue culture;fibroblasts;cell motility

 

数据来源: WILEY

 

摘要:

SUMMARYA method is described which permits the observation of microscopic specimens in both top and side views, a rapid change between the two views being possible without disturbing the specimen. The system requires the introduction of simple optical components into a culture chamber, does not restrict the types of microscopy that may be used, and does not necessitate modification of the microscope itself. The technique is discussed in relation to the study of tissue cells in culture, but may find applications in other areas.

 

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