The first and second derivative spectrophotometric methods have been developed for the determination of flavonoids chrysin and quercetin. The amplitudes in the first derivative spectrophotometric spectra at 256 nm and in the second derivative spectra at 268 nm were selected to determine chrysin, while the amplitudes at 233 and 269 nm in the first and at 241, 258, 387 and 403 nm in the second derivative spectra were chosen for the determination of quercetin. A linear relationship between derivative amplitudes and a concentration of compounds was found. Beer's law is obeyed over a concentration range of 2–20 μg.mL−1for both drug components. The method is rapid, simple, sensitive and does not require a separation step.