The effect of extracellular matrix on the growth of mouse olfactory tissuein vitro
作者:
Gillian Donnelly,
Robert Smith,
Lorna Breckenridge,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 17
页码: 3837-3840
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Collagen gel;Extracellular matrix;Neurite outgrowth;Olfactory bulb;Olfactory sensory neurones;Reconstituted basement membrane
数据来源: OVID
摘要:
THE aim of this study was to compare the capacity of extracellular matrix (ECM) components to support the growth and differentiation of olfactory tissuein vitro. Reconstituted basement membrane matrix (RBM) supported profuse neurite outgrowth from explants of olfactory bulb (OB), with no non-neuronal cell migration. In contrast, nasal tissue (NT) explants cultured on RBM produced profuse fibroblastic outgrowth, but no neurite outgrowth from olfactory sensory neurones (OSN). Three-dimensional collagen gels, on the other hand, supported extensive outgrowth of both nonneuronal and neurite outgrowth from OB and NT explants, mature OSNs in the latter being identified using antibodies against olfactory marker protein (OMP). These culture systems represent useful tools for investigating potential tropic influences of target tissue.
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