Expression of modified low-density lipoprotein receptors by trabecular meshwork cells
作者:
ChangI. L.,
ElnerG.,
YueY. J.T.,
CornicelliA.,
KawaJ. E.,
ElnerV. M.,
期刊:
Current Eye Research
(Taylor Available online 1991)
卷期:
Volume 10,
issue 12
页码: 1101-1112
ISSN:0271-3683
年代: 1991
DOI:10.3109/02713689109024127
出版商: Taylor&Francis
数据来源: Taylor
摘要:
We examined the incooration of fluoresceinated low-density lipoprotein (LDG and acetylated or acetoacetylated low-density lipoprotein (A-LDL or AA-LDL) by a number of ocular cells in culture. All the cells investigated, including bovine, monkey, human trabecular meshwork cells, human corneal endothelial cells, human corneal stromal cells and human scleral cells, took u fluorescently labeled LDL. The bovine, monkey and human trabecular meshwork cells showed the stronest fluorescence reactions. In addition, we found that tae trabecular meshwork cells became fluorescent after incubations with labeled A-LDL or AA-LDL. They were the only cell type examined that possessed this capacity. The fluorescence intensity was markedly diminished by adding to the incubation solution either fucoidin, a competitive inhibitor of modified LDL uptake, unlabeled A-LDL or AA-LDL. The trabecular meshwork cellsin situalso became brightly labeled after exposure to fluoresceinated native LDL, A-LDL or AA-LDL. The uptake of modified LDL separated the trabecular meshwork cells from other types of ocular cells, which may be used to aid identification of trabecular meshwork cells in culture as well asin situ.This proerty also suggested that trabecular meshwork cells may Kave some functional similarities to macrophages.
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