A PCR-based method to amplify DNA with random primers: determining the chromosomal content of porcine flow-karyotype peaks by chromosome painting
作者:
D. Milan,
M. Yerle,
A. Schmitz,
B. Chaput,
M. Vaiman,
G. Frelat,
J. Gellin,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1993)
卷期:
Volume 62,
issue 2-3
页码: 139-141
ISSN:1424-8581
年代: 1993
DOI:10.1159/000133457
出版商: S. Karger AG
数据来源: Karger
摘要:
We present here a new PCR-based technique that allows the production of several micrograms of DNA from only 300 flow-sorted chromosomes. During the first two PCR cycles, the annealing temperature is decreased to 30 °C, and numerous random loci are amplified under nonspecific conditions. As demonstrated here for pig chromosomes 1 and 18, the PCR products may be used to identify the chromosomal content of the flow-karyotype peaks of any species by fluorescence in situ hybridization
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