Cholesteric liquid crystalline DNA; a comparative analysis of cryofixation methods
作者:
Amélie Leforestier,
Françoise Livolant,
期刊:
Biology of the Cell
(WILEY Available online 1991)
卷期:
Volume 71,
issue 1‐2
页码: 115-122
ISSN:0248-4900
年代: 1991
DOI:10.1016/0248-4900(91)90058-U
出版商: Blackwell Publishing Ltd
关键词: liquid crystal;DNA;cryofixation methods;freeze‐fracture;biopolymer
数据来源: WILEY
摘要:
Summary—The ultrastructure of liquid crystalline phases of DNA raises numerous problems because of the structure itself which is fluid and which nature depends on the relative amount of DNA, water and ions. Different cryofixation methods were tested and compared after freeze‐fracture of the specimen. A good ultrastructural preservation of the samples can be achieved without addition of any cryoprotectant by quick‐freezing against a copper block cooled down to liquid helium temperature. Then, molecular orientations can be followed very accurately and the local disorder around a mean direction which exists in the liquid state is kept in the frozen stru
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