Radioreceptor Assay of Opioid Peptides in Selected Canine Brain Regions
作者:
DominicM. Desiderio,
Hisayoshi Takeshita,
期刊:
Analytical Letters
(Taylor Available online 1985)
卷期:
Volume 18,
issue 15
页码: 1789-1798
ISSN:0003-2719
年代: 1985
DOI:10.1080/00032718508062908
出版商: Taylor & Francis Group
关键词: radioreceptor assay;opioids;peptides;brain
数据来源: Taylor
摘要:
A radioreceptor assay using the opioid delta receptor-preferring ligand D-2ala, D-5leu leucine enkephalin (3H-DADL) and the broader-specificity ligand3H-etorphine was used to measure five HPLC-purified neuropeptide fractions derived from the peptide-rich fraction of tissue homogenates of nine anatomical regions of the canine brain. The receptoractive peptides studied were methionine enkephalin, alpha-neo-endorphin, dynorphin 1-8, methionine enkephalin-Arg-Phe, and leucine enkephalin. These peptides derive from two larger precursors: proenkephalin A, which contains methionine enkephalin, leucine enkephalin, methionine enkephalin-Arg-Phe; and proenkephalin B, which contains alpha-neo-endorphin and dynorphin 1-8. Receptoractive peptides were measured in the peptide-rich fraction derived from homogenates of canine hypothalamus, pituitary, caudate nucleus, amygdala, hippocampus, mid-brain, thalamus, pons-medulla, and cortex.
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