Preparation and Use of a Boronic Acid Affinity Support for Separation and Quantitation of Glycosylated Hemoglobins
作者:
A.Krishna Mallia,
GregT. Hermanson,
RandallI. Krohn,
EdwardK. Fujimoto,
PaulK. Smith,
期刊:
Analytical Letters
(Taylor Available online 1981)
卷期:
Volume 14,
issue 8
页码: 649-661
ISSN:0003-2719
年代: 1981
DOI:10.1080/00032718108055476
出版商: Taylor & Francis Group
关键词: Glycosylated Hemoglobins;boronate-/ /-agarose;affinity chromatography;diabetes mellitus
数据来源: Taylor
摘要:
Cross-linked agarose activated with carbonyl diimidazole and subsequently coupled to aminophenyl boronic acid provides a highly efficient matrix for separation and quantitation of glycosylated hemoglobins The method described is highly reproducible. rapid and flexible in that it can be adapted to column or batch formats The method can tolerate moderate fluctuations in temperature and pH of buffeused without significantly altering results Hemoglobin fractions separated by this affinity support were Subjected to isoelectric focusing which revealed a heterogeneous population of glucose modified proteins.
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