Nonparenchymal Cells Cultivated from Explants of Fibrotic Liver Resemble Endothelial and Smooth Muscle Cells from Blood Vessel Walls
作者:
Bruno Voss,
Jürgen Rauterberg,
Gerhard Pott,
Ute Brehmer,
Salah Allam,
Rolf Lehmann,
Dirk B. V. Bassewitz,
期刊:
Hepatology
(WILEY Available online 1982)
卷期:
Volume 2,
issue 1
页码: 19-28
ISSN:0270-9139
年代: 1982
DOI:10.1002/hep.1840020105
出版商: W.B. Saunders
数据来源: WILEY
摘要:
AbstractTissue specimens from human fibrotic liver obtained by needle biopsy were cultured. Two cell types emerged from the tissue explants. From their morphology and biosynthetic products they resembled smooth muscle cells and endothelial cells from blood vessel walls. In the “endothelial” cells, factor VIII‐associated protein was demonstrated by indirect immunofluorescence. Synthesis of collagen types I and III, basement membrane collagen types IV and V, and fibronectin by both cell types was observed by immunofluorescence microscopy. Homogeneous cultures of “smooth muscle cells” were observed in subcultures. After incubation with [14C]glycine, collagen was isolated and characterized by CM cellulose chromatography, and consisted mainly of types I and III. These data suggest involvement of mesenchymal cells in hepatic fibrosis; they presumably originate from blood vessel or sinusoi
点击下载:
PDF
(901KB)
返 回