BIOCHEMICAL AND FUNCTIONAL CHARACTERIZATION OF SOLUBLE MULTIVALENT MHC Ld/Fcγ1 AND Ld/Fcμ CHIMERIC PROTEINS LOADED WITH SPECIFIC PEPTIDES1
作者:
Lepley2 Denise,
Gillanders3 William,
Myers3 Nancy,
Robinson4 Ruth,
Beisel2 Kirk,
Wisecarver2 James,
Pirruccello2 Samuel,
Lee4 David,
Hansen3 Ted,
Rubocki2,5 Ronald,
期刊:
Transplantation
(OVID Available online 1997)
卷期:
Volume 63,
issue 5
页码: 765-774
ISSN:0041-1337
年代: 1997
出版商: OVID
数据来源: OVID
摘要:
Central to the specificity of the immune system is the interaction between the T cell receptor and the major histocompatibility complex (MHC)-peptide ligand complex. To better understand the nature of this interaction, and to investigate possible avenues for specific therapeutic intervention, we have produced soluble recombinant molecules that can modulate antigen-specific T cells. Our approach involved the construction of recombinant murine genes composed of the MHC class I geneH-2Ldand the Fc portion of immunoglobulin (Ig) heavy chain genes μ or γ1. Stable transfectants of theseLd/Fcγ1andLd/Fcμ genes generated correctly spliced transcripts and were capable of secreting chimeric protein. Immunoprecipitation analyses demonstrated the presence of chimeric Ld/Fcγ1 and Ld/Fcμ monomers of approximately 69 kDa and 90 kDa, respectively, as well as chimeric dimers under nonreducing conditions. The capacity of Ld/Ig molecules to bind specific peptide ligands was demonstrated using radiolabeled peptides or with monoclonal reagents that specifically identify peptide-induced conformational changes in the Ldligand binding site. Soluble divalent Ld/Fcγ1 molecules were loaded with the murine cytomegalovirus-derived peptide and other Ld-specific peptide ligands and subsequently isolated and purified. Peptide-loaded Ld/Fcγ1 molecules were capable of inhibiting the response of class I-restricted T cells in vitro in a peptide-specific fashion. The development of soluble multivalent chimeric proteins that possess unique properties of both the MHC class I and Ig molecules provides a valuable reagent for the study of potential mechanisms of in vitro and in vivo immune modulation.
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