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Structure of the Blend Membrane of Regenerated Silk Fibroin and Glucose Oxidase and Its Application to Glucose Sensor

 

作者: Xiaohua Zhang,   Haiying Liu,   Yongcheng Liu,   Jiaqi Deng,   Tongying Yu,  

 

期刊: Analytical Letters  （Taylor Available online 1995）
卷期: Volume 28, issue 9  

页码: 1593-1609

 

ISSN:0003-2719

 

年代: 1995

 

DOI:10.1080/00032719508002767

 

出版商: Taylor & Francis Group

 

关键词: Regenerated silk fibroin;Glucose oxidase;Glucose;Membrane;Sensor

 

数据来源: Taylor

 

摘要:

Regenerated silk fibroin was successfully used to immobilize glucose oxidase and the structure of the blend membrane was investigated. The molecules of glucose oxidase in the membrane were in aggregates. Glucose sensor was fabricated by coupling the blend membranes with Clark electrode. Its kinetic constants such as apparent Michael's constants Kmapp, maximal current response ΔSmaxand apparent activation energies Ea of the reaction, effects of pH, temperature and ethanol on the sensor were examined.

 

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