Platelet Adhesivity to Subendothelium Is Influenced by Polymorphonuclear Leukocytes: Studies with Aspirin and Salicylate
作者:
Rosa Hernández,
Mónica Alemany,
Jordi Bozzo,
Michael R. Buchanan,
Antonio Ordinas,
Eva Bastida,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1993)
卷期:
Volume 23,
issue 1
页码: 1-7
ISSN:1424-8832
年代: 1993
DOI:10.1159/000216845
出版商: S. Karger AG
关键词: Platelets;Polymorphonuclear leukocytes;Leukocytes;Vascular subendothelium;Aspirin;Salicylate;Metabolites;Blood flow
数据来源: Karger
摘要:
Interactions between blood platelets and polymorphonuclear leukocytes (PMN) influence cell reactivity. We have examined the effects of inhibiting platelet and PMN cyclooxygenase and lipoxygenase pathways, by treatment with aspirin (ASA) and salicylate (SAL) on platelet-vessel wall interaction studied under flow conditions by means of an annular chamber perfusion system. We have also measured the levels of cyclooxygenase-derived metabolites during perfusion. Perfusates were prepared with untreated and ASA- or SAL-treated PMN or platelets. Our results demonstrated that blockage of the lipoxygenase pathway in PMN significantly increased platelet thrombus formation and favored the production of thromboxane B2 (T×B2) during perfusion, whereas inhibition of cyclooxygenase pathway in PMN had no effect either on platelet deposition or on T×B2 levels. In contrast, blockage of platelet cyclooxygenase, which caused almost total inhibition of T×B2, enhanced platelet adhesion and did not modify platelet thrombus formation. These results suggest that under dynamic conditions cooperative metabolic mechanisms between platelets and PMN directly influence platelet interaction with vascular subendotheli
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