Serine Proteinase Inhibitors Produced by Human Melanoma Cell Lines
作者:
Dario Giacomoni,
Feridoon Najmabadi,
Sheldon Dray,
期刊:
Tumor Biology
(Karger Available online 1990)
卷期:
Volume 11,
issue 1-2
页码: 39-50
ISSN:1010-4283
年代: 1990
DOI:10.1159/000217641
出版商: S. Karger AG
关键词: Melanoma;human;Serine proteinase inhibitors
数据来源: Karger
摘要:
The human melanoma cell lines M21 and MSM-M2 are shown to produce two similar competitive inhibitors of trypsin, a serine proteinase. These proteinase inhibitors inhibited the serine proteinases trypsin and kallikrein with similar efficiency but did not inhibit plasmin (a serine proteinase) or papain (a thiol proteinase). Active synthesis of the inhibitors during cell culture was indicated by the requirement for cell viability, the increase in inhibitory activity of the supernatant with time, and the incorporation of 35S-methionine into the inhibitors. The two inhibitors were stable to heat (70 °C) and extremes of pH. Their molecular weights were estimated at 670 and 250 kD, respectively. A screening of the super-natants of five other human melanoma cell lines by HPLC showed that they all released a similar trypsin inhibitory factor not detected in human or bovine serum. The isolation of these proteinase inhibitors facilitates a study of their putative role in tumor growth
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