Blocking of Non-Specific Sorption in Elisa on Formylated Polystyrene Beads
作者:
VadimV. Shmanai,
期刊:
Journal of Immunoassay
(Taylor Available online 1999)
卷期:
Volume 20,
issue 1-2
页码: 13-30
ISSN:0197-1522
年代: 1999
DOI:10.1080/01971529909349311
出版商: Taylor & Francis Group
关键词: Elisa;solid phase;polystyrene beads;covalent immobilization;non-specific sorption;peroxidase conjugates
数据来源: Taylor
摘要:
The effect of pH of the blocking solution on the non-specific sorption of peroxidase conjugates to the 0.63-cm formylated polystyrene beads was studied. Anti-G-HRP and BSA-HRP sorption was shown to dramatically depend on pH of blocking protein solutions and was minimum at pH=pI. Sorption of G-HRP weakly depended on pH of the blocking buffer. the blocking efficiency of proteins on unmodified beads, as well as of Tween-20 on formylated beads appeared to be pH independent. the optimized blocking step resulted in a multiple decrease in the non-specific sorption of conjugates on formylated beads.
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