Human choroidal melanocytes in tissue culture
作者:
WaldrepJ. Clifford,
KaplanHenry J.,
期刊:
Current Eye Research
(Taylor Available online 1986)
卷期:
Volume 5,
issue 8
页码: 587-594
ISSN:0271-3683
年代: 1986
DOI:10.3109/02713688609015123
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Human choroidal melanocytes were isolated from eye bank tissue and cultured in vitro with 10ng/ml cholera toxin and 10ng/ml phorbol myristate acetate. These growth factors induced rapid proliferation of spindle-type melanocytes. Similar results were obtained using 10mM putrescine. Phenotypic analysis of these cells by immunoperoxidase staining with anti-HLA-DR monoclonal antibodies revealed 40-70% positive cells. HLA-DR positive melanocytes were isolated and purified from the HLA-DR negative population by resetting with protein-A coupled sheep erythrocytes followed by differential centrifugation. Ultrastructural analysis of choroidal melanocytes revealed evidence of premelanosomes and melanosomes. Choroidal melanocytes in tissue culture are important experimental controls for the study of the cell biology of choroidal melanoma.
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