A Monoclonal Antibody that Neutralizes Epstein-Barr Virus DNA Polymerase Activity
作者:
Ching-Hwa Ann Tsai,
Marshall V. Williams,
Ronald Glaser,
期刊:
Intervirology
(Karger Available online 1990)
卷期:
Volume 31,
issue 2-4
页码: 215-222
ISSN:0300-5526
年代: 1990
DOI:10.1159/000150156
出版商: S. Karger AG
关键词: Epstein-Barr virus;Monoclonal antibody;DNA polymerase;Neutralization
数据来源: Karger
摘要:
An Epstein-Barr virus (EBV) specific monoclonal antibody (MAb), designated 55H3, was produced after immunizing BALB/c mice with 12–0-tetradecanoylphorbol-13-acetate (TPA) activated B95–8 cells. It was possible to demonstrate that this MAb detected an EBV-specific antigen(s) in the EBV genome-positive producer cell lines B95–8 and M-AB A by immunofluorescence. Immunoglobulin prepared from ascites fluid neutralized the activity of the EBV-encoded DNA polymerase, but not the alkaline DNase. By Western blotting, the 55H3 MAb reacts with an 85/80-KD polypeptide. The 55H3 should be useful in examining the role of EBV DNA polymerase in viral replic
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