Apoptosis and expression of DNA repair proteins in ischaemic brain injury in man
作者:
Seth Love,
Rachel Barber,
Gordon Wilcock,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 6
页码: 955-959
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Apoptosis;Brain ischaemia;Cardiac arrest;DNA repair;Hypoxia;In situend-labelling;Ku80;Poly(ADP-ribose) polymerase;Stroke
数据来源: OVID
摘要:
We examined the timing of apoptosis and the expression of the DNA repair proteins poly(ADP-ribose) polymerase (PARP) and Ku80 in sections of frontal and temporal lobes from patients who had suffered severe brain ischaemia due to a cardiac arrent.In situ endlabelling(ISEL) was used to detect apoptotic cells, and immunohistochemistry to assess PARP and Ku80. ISEL of scattered neurons and glia was demonstrable predominantly during the first 24 h after ischaemia. PARP and Ku80 immunoreactivity increased markedly after cerebral ischaemia, PARP particularly in the regions of greatest susceptibility to hypoxic injury: the CA1 field of the hippocampus and the depths of noecortical sulci. The up-regulation of PARP is in keeping with experimental observations concerning the key role of this enzyme in mediating ischaemic cell death.
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