The effects of pyruvate, succinate, citrate, alpha‐keto glutarate, fumarate, malate, lactate, and glycerol supplementation (0.5, 1.0, and 2.0 g/100 ml) on aflatoxin production by A. parasiticus NRRL 2999 were examined in synthetic and semi‐synthetic media. Glycerol, lactate, and the 0.5 g/100 ml level of pyruvate stimulated production in the synthetic medium. At the 2.0 g/100 ml level, pyruvate, citrate, alpha‐keto glutarate, fumarate, and malate inhibited aflatoxin accumulation in both media. With the exception of glycerol, all supplements stimulated sporulation and elevated the pH of the cul