HIV‐1 biological phenotype in long‐term infected individuals evaluated with an MT‐2 cocultivation assay
作者:
Maarten Koot,
Aster Vos,
Rene Keet,
Ruud de Goede,
M. Dercksen,
Fokke Terpstra,
Roel Coutinho,
Frank Miedema,
Matthijs Tersmette,
期刊:
AIDS
(OVID Available online 1992)
卷期:
Volume 6,
issue 1
页码: 49-54
ISSN:0269-9370
年代: 1992
出版商: OVID
关键词: HIV-1;AIDS;pathogenesis;HIV-1 biological phenotype;syncytium induction;virus isolation;T-cell lines.
数据来源: OVID
摘要:
ObjectiveWe have previously demonstrated that detection of syncytium-inducing (SI) HIV-1 in asymptomatic seropositive individuals is associated with rapid progression to AIDS. In the present study, we sought to develop and evaluate an HIV-1 phenotyping assay for the screening of large numbers of individuals.MethodsEfficiency of HIV-1 isolation from patient peripheral blood mononuclear cells (PBMC) was studied with donqr PBMC or seven different CD4 + T-cell lines as target cells. The biological phenotype of sequential isolates from 20 long-term asymptomatic HIV-1-seropositive individuals was determined by two different assays.ResultsNon-SI isolates, efficiently recovered by cocultivation with donor PBMC, were never isolated with T-cell lines as target cells. Direct cocultivation with MT-2 cells, but not with six other CD4+ T-cells, resulted in the efficient recovery of SI isolates. HIV-1 MT-2 tropism and SI capacity were shown to be coupled properties at the clonal level. SI isolates emerged in 10 out of 20 longitudinally-studied individuals. In these long-term infected individuals, appearance of SI isolates was associated with progression to AIDS.ConclusionsDirect cocultivation of patient PBMC with the MT-2 cell line is a sensitive, specific and convenient method to detect SI isolates. The availability of an assay suitable for the screening of large groups allows further study of the value of HIV-1 biological phenotyping as a prognostic marker.
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