Isolation and Identification of a Mannolipid Intermediate in the Synthesis of Pulmonary Glycoproteins
作者:
PasseroMichael A.,
PassConstance W.,
HutchinsonThomas R.,
期刊:
Experimental Lung Research
(Taylor Available online 1981)
卷期:
Volume 2,
issue 1
页码: 17-26
ISSN:0190-2148
年代: 1981
DOI:10.3109/01902148109052299
出版商: Taylor&Francis
数据来源: Taylor
摘要:
In order to demonstrate the role of lipid-linked sugars as intermediates in the synthesis of pulmonary glycoproteins, membranous preparations of rabbit lung were incubated with GDP-14C-mannose and selectively extracted with chloroform-methanol (2:1) and chloroform-methanol-water (1:1.03). The chloroform-methanol extract was found to contain a lipid-linked sugar which had chemical and chromatographic properties similar to mannosyl-phosphoryl dolichol. The chloroform-methanol-water extract contained an oligosaccharide- lipid and the remaining precipitate contained a labeled protein. Transfer of mannose from the labeled mannolipid to the endogenous protein was demonstrated. These studies indicate a glycolipid intermediate is present in the synthesis of oligosaccharides attached to pulmonary glycoproteins.
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