首页   按字顺浏览 期刊浏览 卷期浏览 Determination of Pyruvate Kinase and Creatine Kinase Using Multienzyme Electrodes
Determination of Pyruvate Kinase and Creatine Kinase Using Multienzyme Electrodes

 

作者: D. Weigelt,   F. Schubert,   F. Scheller,  

 

期刊: Analytical Letters  （Taylor Available online 1988）
卷期: Volume 21, issue 2  

页码: 225-239

 

ISSN:0003-2719

 

年代: 1988

 

DOI:10.1080/00032718808055748

 

出版商: Taylor & Francis Group

 

关键词: Pyruvate kinase;creatine kinase;biosensor clinical analysis

 

数据来源: Taylor

 

摘要:

The application of a pyruvate-sensing bienzyme electrode based on lactate dehydrogenase, lactate mono-oxygenase and an oxygen probe for pyruvate kinase (PK) determination in erythrocytes is described. PK can be measured in the range 0.12 – 1.85 μmol/(s1) with good reproducibility and good agreement with the conventional method. Coimmobilization of PK in the enzyme membrane leads to asensor for creatine kinase determination useful in the range 0.1 – 1.76 μmol/(s1).

 

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