Measurement of IgE on Rat Mast Cells: Relation to Serum IgE and Allergen‐Induced Histamine Release
作者:
A.W. TOORENENBERGEN,
P. SWIETEN,
R. C. AALBERSE,
期刊:
Scandinavian Journal of Immunology
(WILEY Available online 1983)
卷期:
Volume 17,
issue 1
页码: 13-18
ISSN:0300-9475
年代: 1983
DOI:10.1111/j.1365-3083.1983.tb00760.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
IgE antibodies against trinitrophenylated ovalbumin (TNP‐OVA) were raised in Brown Norway rats by a highly reproducible immunization procedure. With these rats, the kinetics of the IgE response was studied, both for free circulating and cell‐bound IgE. Cell‐bound IgE was dissociated from peritoneal cell suspensions at acid pH; TNP‐specific and total IgE in serum and in eluates of peritoneal cells were determined by radioimmunoassay. Both for total and TNP‐specific IgE, a significant correlation was found between the serum IgE level and the amount of IgE molecules per mast cell. One day before TNP hapten could induce histamine release from mast cells, TNP‐specific IgE appeared in the circulation and on the cells. These results suggest that also in active sensitization, there is a lag time for sensitization of
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