Binding of Protoporphyrin to Hemoglobin in Red Blood Cells of Patients with Erythropoietic Protoporphyria
作者:
van SteveninckJ.,
DubbelmanT. H. A. R.,
de GoeijA. F. P. M.,
WentL. N.,
期刊:
Hemoglobin
(Taylor Available online 1977)
卷期:
Volume 1,
issue 7
页码: 679-690
ISSN:0363-0269
年代: 1977
DOI:10.3109/03630267708999174
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Virtually all protoporphyrin in erythrocytes of patients vith erythropoietic protoporphyria is bound to hemoglobin. The maximum of the fluorescence excitation spectrum of this protoporpliy-rin-hemoglobin complex shifted, with increasing concentration, from 405 nm to 389 nm. A similar shirt was observed wlien titrating a solution of free protoporphyrin with hemoglobin. The Soret maximum of free protoporphyrin itself. on the other hand, was not concentration-dependent. These observations indicate that spectrofluorometric measurements do not allow conclusions coticerning the mode of protoporphyrin binding to hemoglobin.Experiments on protoporphyrin exchange between the hemoglobins A, F and S reinforced the previously drawn conclusion that protoporphyrin is bound to hemoglobin at the heme-binding sites.
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