Capsules for Frozen Sectioning of Small Specimens
作者:
LeemanA. L.,
NicholsonP. W.,
DeshmukhA. A.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 1
页码: 14-16
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110542
出版商: Taylor&Francis
关键词: frozen sectioning
数据来源: Taylor
摘要:
It was necessary to make sections of small unfixed specimens which had been frozen while still immersed in their normal culture medium. The principal difficulty stemmed from the poor sectioning quality of the frozen culture medium. A capsule is described which has a narrow well in which the tissue specimen fits snugly within a small amount of culture medium. After freezing, the whole capsule is sectioned and the resulting sections, being nearly devoid of culture medium, are of good quality.
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