High‐resolution electron microscopy studies as well as computer simulation studies on oxygen vacancies in Y‐Ba‐Cu‐O superconductors show that the existence of oxygen vacancies at {0, 1/2, 0} sites leads to the distortions of structural images. For certain imaging conditions, the spots corresponding to the column of oxygen vacancies can be distorted heavily, so that the indirect image of oxygen vacancies in Y‐Ba‐Cu‐O superconductors may be acquired. The results reveal that even in local area the oxygen vacancies may be distributed regularly.