Regional calpain and caspase‐3 proteolysis of α‐spectrin after traumatic brain injury
作者:
Brian Pike,
Xiurong Zhao,
Jennifer Newcomb,
Rand Posmantur,
Kevin Wang,
Ronald Hayes,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 11
页码: 2437-2442
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Apoptosis;Cell death;CNS;CPP32;Cytoskeleton;Fodrin;Ischemia;Necrosis;Neuron
数据来源: OVID
摘要:
ACTIVITY of calpains and caspase-3 inferred from proteolysis of the cytoskeletal protein α-spectrin into signature spectrin breakdown products (SBDPs) was used to provide the first systematic and simultaneous comparison of changes in activity of these two families of cysteine proteases after traumatic brain injury (TBI) in rats. Distinct regional and temporal patterns of calpain/caspase-3 processing of α-spectrin were observed in brain regions ipsilateral to the site of injury after TBI, including large increases of 145 kDa calpain-mediated SBDP in cortex (up to 30-fold), and enduring increases (up to 2 weeks) of 145 kDa SBDP in hippocampus and thalamus. By contrast, 120 kDa caspase-3-mediated SBDP was absent in cortex and showed up to a 2-fold increase in hippocampus and striatum at early (hours) after TBI. Future studies will clarify the pathological significance of large regional differences in activation of calpain and caspase-3 proteases after TBI.
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