Allyl alcohol cytotoxicity in isolated rat hepatocytes: Effects of azide, fasting, and fructose
作者:
LoraE. Rikans,
Yong Cai,
K. Roger Hornbrook,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1995)
卷期:
Volume 44,
issue 1
页码: 1-11
ISSN:0098-4108
年代: 1995
DOI:10.1080/15287399509531939
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The role of altered energy homeostasis in the lethality of allyl alcohol to isolated rat hepatocytes was studied. ATP, ADP, AMP, and viability loss (leakage of lactate dehydrogenase into the medium) were measured in isolated hepatocytes of fed or fasted rats exposed to 0.5 mM allyl alcohol. Adenine mononucleotides and cytotoxicity were determined also in hepatocytes incubated with allyl alcohol in the presence of 4 mM sodium azide or 15 mM fructose. Allyl alcohol‐induced cell death in hepatocytes of fed rats was preceded by slight decreases in ATP content and energy charge (16% and 12%, respectively). More substantial decreases in these parameters occurred in parallel with cell killing, but the effect of allyl alcohol on energy status did not exceed the effect produced by a nonlethal concentration of sodium azide. Neither azide nor fructose affected the development of allyl alcohol cytotoxicity. Moreover, allyl alcohol‐induced cytotoxicity was similar in hepatocytes of fed and fasted rats. The results suggest that altered energy homeostasis is a consequence rather than a cause of allyl alcohol‐induced hepatocyte lethality.
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