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Spectrofluorimetric Determination of Nitrite with Pyridoxal-5-Phosphate-2-Pyridylhydrazone

 

作者: S. Rubio,   A. Gómez-Hens,   M. Valcárcel,  

 

期刊: Analytical Letters  （Taylor Available online 1984）
卷期: Volume 17, issue 8  

页码: 651-663

 

ISSN:0003-2719

 

年代: 1984

 

DOI:10.1080/00032718408069092

 

出版商: Taylor & Francis Group

 

关键词: Spectrofluorimetry;nitrite;pyridoxal-5-phosphate-2-pyridylhydrazone

 

数据来源: Taylor

 

摘要:

The nitrite ion oxidizes pyridoxal-5-phosphate-2-pyridyl-hydrazone in acid medium giving a fluorescent product (λex325 nm, λem420 nm). This redox reaction is used to developed a spectrofluorimetric method for the determination of nitrite. The calibration graph is liner in the 0.1 − 1.0 μg mL−1range. The interference levels, stoichiometry and nature of the reaction have been studied. The method is applied to determine nitrite in water and soil samples

 

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