CENTRIFUGAL PRECIPITATION CHROMATOGRAPHY APPLIED TO FRACTIONATION OF PROTEINS WITH AMMONIUM SULFATE
作者:
Yoichiro Ito,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 18
页码: 2825-2836
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100102062
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A novel chromatographic system introduced here internally generates a concentration gradient of ammonium sulfate (AS) along a long channel to fractionate proteins according to their solubility in AS solution. The separation column consists of a pair of discs with mutually mirror-imaged spiral channels that are separated by a semipermeable membrane. The disc assembly is mounted on a seal-less continuous flow centrifuge. A concentrated AS solution is introduced into the upper channel while a water solution is passed through the lower channel in the opposite direction in a rotating column. A mixture of proteins injected into the water channel moves along an AS gradient of increasing concentration that has been established in the water solution. Each protein species precipitates at a different AS concentration along the gradient. By decreasing the concentration of the AS solution its concentration in the water is decreased, causing the protein to redissolve and to reprecipitate further down the channel. The eluate is continuously monitored and collected using a fraction collector.
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