Vegetative riparian buffer strips are typically used to curb stream degradation due to cattle grazing, but intensive rotational grazing has shown promise as an alternative best management practice. We compared aquatic macroinvertebrate assemblages among stream segments within continuously grazed pastures, intensive rotationally grazed pastures, undisturbed grassy vegetative buffer strips, and undisturbed woody vegetative buffer strips. We collected macroinvertebrate and stream sedimentation data from four streams in each land use category in two consecutive years. In an attempt to account for inherent watershed variability among streams, we represented watershed condition with a sample collected upstream of each treatment reach. Watershed condition tended to have greater influence on macroinvertebrate measures than local riparian land use. However, local riparian land use influences were apparent if watershed condition was statistically accounted for with analysis of covariance. Stream reaches with intensive rotational grazing tended to have macroinvertebrate assemblage characteristics intermediate of the buffer and continuously grazed reaches. Although we detected some differences in macroinvertebrate assemblages that apparently reflected very local land use, our results suggest the macroinvertebrates were mostly responding to large-scale watershed influences.