Fluorescent localization of calcium at sites of cell attachment and spreading
作者:
Denis Moran,
期刊:
Journal of Experimental Zoology
(WILEY Available online 1984)
卷期:
Volume 229,
issue 1
页码: 81-89
ISSN:0022-104X
年代: 1984
DOI:10.1002/jez.1402290111
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractChlorotetracycline (CTC), a calcium‐chelating fluorescent probe identifies and localizes calcium when applied to cultured neural crest cells. The fluorescence occurs at specific membrane regions involved in cell attachment and spreading as well as with the formation of cytoskeletal stress fibers (actin microfilament bundles). The observed CTC reaction indicates that calcium sequestration and the development of these membrane‐cytoskeletal features share a temporal and spatial pattern. Thus, the selective availability of calcium may be an important determinant of cell morphogene
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