A simple fluorescence technique for light microscopy of the crystalline lens
作者:
RaeJ. L.,
TruittK. D.,
KuszakJ. R.,
期刊:
Current Eye Research
(Taylor Available online 1982)
卷期:
Volume 2,
issue 1
页码: 1-5
ISSN:0271-3683
年代: 1982
DOI:10.3109/02713688208998374
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Lenses fixed in formaldehyde or glutaraldehyde can be embedded in plastic, and sections as large as 3–4 mm across can be cut with glass knives on an ultramicrotome. When these sections are stained with 25% sodium fluorescein and allowed to bleach in a fluorescence microscope, high contrast images of essentially every cell in the section, including cells very near the lens center, can be seen. The method is effective with lenses from a number of species, and should be useful for a wide variety of morphological studies of normal and cataractous lenses.
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