Evidence for reduced lipid order in plasma membranes from cataractous human lenses
作者:
GoodenMarty M.,
TakemotoLarry J.,
RintoulDavid A.,
期刊:
Current Eye Research
(Taylor Available online 1982)
卷期:
Volume 2,
issue 6
页码: 367-375
ISSN:0271-3683
年代: 1982
DOI:10.3109/02713688209000782
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Membrane preparations from normal and cataractous human lenses were prepared by a procedure which minimally disrupts membrane lipid structure. Fluorescence depolarization ofcisandtrans-parinaric acid probes was measured as a function of temperature in membranes, lipid extracts, and phospholipids. These measurements indicated that membranes from cataractous lenses were less ordered (more fluid) than equivalent membranes from normal, age-matched control lenses. No significant differences could be detected between normal and cataractous membrane total lipid extracts, or between normal and cataractous membrane phospholipids. These observations imply that membrane lipid-protein interactions play a significant role in determination of lens membrane structure, and that these interactions are altered in cataractous lens membranes.
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