The distribution and relative potency of diuretic peptides in the house cricket, Acheta domesticus
作者:
G. M. COAST,
C. H. WHEELER,
期刊:
Physiological Entomology
(WILEY Available online 1990)
卷期:
Volume 15,
issue 1
页码: 13-21
ISSN:0307-6962
年代: 1990
DOI:10.1111/j.1365-3032.1990.tb00487.x
出版商: Blackwell Publishing Ltd
关键词: Diuretic peptides;peptide distribution;Malpighian tubule fluid secretion;Acheta domesticus.
数据来源: WILEY
摘要:
ABSTRACTDose‐response curves are presented for the diuretic activity in aqueous extracts of brain, retrocerebral complex, and ventral nerve cord ganglia fromAcheta domesticus. Diuretic activity is highest in extracts of brain and corpora cardiaca. In comparison with such extracts, those of the suboesophageal ganglia and thoracic ganglia I‐III produce truncated responses, whilst abdominal ganglia 1–4 show evidence of an inhibition of the diuretic response at high doses. ED50values, obtained from Hill plots, are similar for extracts of brain, corpora cardiaca, corpora allata, and abdominal ganglia, but are 3–4 times higher for extracts of suboesophageal and thoracic ganglia.Separation of aqueous extracts of corpora cardiaca by reversed‐phase HPLC yields a number of fractions which stimulate fluid secretion in isolated tubules. Diuretic activity in these fractions is destroyed by treatment with Pronase E, and on this basis is identified as peptidic. In general, diuretic activity is found in the same RP‐HPLC fractions prepared from aqueous extracts of brain, suboesophageal ganglia, thoracic ganglia I‐III, and abdomina
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