AN AUTOMATED ASSAY OF MEROZOITE INVASION OF ERYTHROCYTES USING HIGHLY SYNCHRONIZEDPLASMODIUM FALCIPARUMCULTURES
作者:
Peter Myler,
Allan Saul,
Thomas Mangan,
Chev Kidson,
期刊:
Australian Journal of Experimental Biology and Medical Science
(WILEY Available online 1982)
卷期:
Volume 60,
issue 1
页码: 83-89
ISSN:0004-945X
年代: 1982
DOI:10.1038/icb.1982.6
出版商: Nature Publishing Group
数据来源: WILEY
摘要:
SummaryPlasmodium falciparumcultures were synchronized using three lytic treatments with sorbitol. Schizonts from these cultures were used in a rapid, highly sensitive assay of invasion of erythrocytes by merozoites. The parasitaemias in recipient cells after invasion were determined by flow cytofluorimetry after staining with the dye 33258 Hoechst. Invasion of erythrocytes was shown to be reduced by serum from a patient with malaria. The assay is suitable for rapidly screening large numbers of samples, such as monoclonal antibodies.
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