Improved Method for Studying Skin Lipid Samples from Cyanoacrylate Strips by High-Performance Thin-Layer Chromatography
作者:
Eva-Maria Röpke,
Wolfgang Augustin,
Harald Gollnick,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1996)
卷期:
Volume 9,
issue 6
页码: 381-387
ISSN:1660-5527
年代: 1996
DOI:10.1159/000211449
出版商: S. Karger AG
关键词: Skin surface lipids;Cyanoacrylate stripping;High-performance thin-layer chromatography
数据来源: Karger
摘要:
A non-invasive method for sampling skin surface lipids is cyanoacrylate stripping (CAC-TS). It was the purpose of our study to improve the method for sampling of skin surface lipids and the separation of epidermal lipid fractions by a modification of the methods described by Melnik and by Imokawa et al. Briefly, lipids on the glass slide sampled by CAC-TS from the forearm of 75 volunteers and from the forehead of 60 volunteers were eluted in hexane/ethanol under ultrasonication. Identification of the diluted total superficial sebaceous and epidermal skin lipids was performed by sequential high-performance thin-layer chromatography. For quantification of the lipids we used densitometric methods. By this modified method we were able to show a clear and complete separation of all relevant lipids from a cyanoacrylate strip that represented 1-2 mg stratum corneum only.
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