The appraisal of an automated multi‐immunoaffinity chromatography system to detect anabolic agents in bile and urine
作者:
TerenceL. Fodey,
ChristopherT. Elliott,
StevenR. H. Crooks,
WilliamJ. Mccaughey,
期刊:
Food and Agricultural Immunology
(Taylor Available online 1996)
卷期:
Volume 8,
issue 3
页码: 157-167
ISSN:0954-0105
年代: 1996
DOI:10.1080/09540109609354914
出版商: Taylor & Francis Group
关键词: Automation;immunoaffinity;anabolic;bile;urine
数据来源: Taylor
摘要:
Screening for residues of anabolic steroids frequently requires extraction from tissues and fluids before analysis. Chemical procedures for these extractions can be complicated, expensive to perform and not ideal for the simultaneous extraction of analytes with different solubilities. Extraction by multi‐immunoaffinity chromatography (MIAC) may be used as an alternative. Samples are passed through a column containing a range of antibodies immobilized on an inert support. The desired analytes are bound to their respective antibodies, washed and then eluted by a suitable solvent The purified extracts can then be incorporated into the analytical tests. The analytes that can be extracted presently are α‐nortestosterone, zeranol, trenbolone, diethylstilboestrol, boldenone and dexamethasone. Manually, the MIAC procedure is limited to about six columns per operator but by automating the process using a robotic sample processor (RSP), 48 columns can be run simultaneously during the day or night. The RSP has also been adapted to transfer extracts and reagents on to ELISA plates. The automated system has proved to be a robust and reliable means of screening large numbers of samples for anabolic agents with minimal manual input.
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