The distribution and excretion of hexachlorobenzene (HCB) after administration to quails of a single oral dose of 4.5 Ci[14C]HCBper l00g body weight was studied by whole‐body autoradiography and liquid scintillation counting. Radiolabeled HCB was distributed throughout the body in 2 h. Peak levels were found 4–8 d after administration, with the highest concentrations in the subcutaneous and abdominal fat, uropygialgland, bone marrow, adrenals, ovarian stroma, and liver, in that order. After 3 wk, substantial amounts of radiolabeled substance were present only in the adipose tissue and the uropygial gland. The main routes of excretion were the egg yolk, uropygial gland, and bile. About 75% of the administered dose was excreted through the egg yolk and about 25% in the feces.