Crystalline sucrose has been isolated from cleared alcoholic extracts of mature wheat leaves by precipitation as the barium salt. The crystals were positively identified by melting point, X-ray diffraction analysis, and glucose–fructose ratio as well as by conventional analytical techniques. The evidence for the presence of sucrose in the wheat leaf has been corroborated by chromatographic analysis of leaf extracts. It is concluded that sucrose forms the bulk of the soluble sugar reserve of this tissue.