Regulation of Macrophage Colony-Stimulating Factor (M-CSF) Production in Cultured Human Synovial Fibroblasts
作者:
HamiltonOhn a.,
FilonziEnrico l.,
IanchesGina,
期刊:
Growth Factors
(Taylor Available online 1993)
卷期:
Volume 9,
issue 2
页码: 157-165
ISSN:0897-7194
年代: 1993
DOI:10.3109/08977199309010831
出版商: Taylor&Francis
关键词: macrophage-colony stimulating factor;synovial cells
数据来源: Taylor
摘要:
AbstractObjective. To study the regulation of macrophage-colony stimulating factor (M-CSF) formationin vitroby human synovial fibroblast-like cells.Methods. Human synovial cell explant cultures were established using cells from non-rheumatoid donors. M-CSF antigen was measured by immunoassay, and messenger RNA (mRNA) levels were determined by Northern blot.Results. The cytokines, interleukin-1 (IL-1), tumor necrosis factora (TNFα), interferon-γ(IFN-γ) and IL-4, increased production of M-CSF above constitutive levels. The presence of the cyclooxygenase inhibitor, indomethacin, potentiated the action of IL-1 on M-CSF synthesis, suggesting that an endogenous cydooxygenase product(s) can down-regulate M-CSF formation. Changes in M-CSF mRNA levels paralleled those in protein levels. The glucocorticoid, dexamethasone, and the retinoid, all-trans retinoic acid, stimulated M-CSF formation. The control of M-CSF synthesis in the synovial fibroblasts differs from that for granulocyte macrophage-CSF (GM-CSF) and granulocyte-CSF (G-CSF).Conclusion. These results suggest that cytokine-stimulated synovial fibroblasts may be a source of M-CSF production in the joints of patients with inflammatory arthritis; as a result, monocyte/macrophages may be activated, leading to perpetuation of the inflammation and destructive events occurring in these lesions.
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