Chromosomal Analysis of Purified B-Chronic Lymphocytic Leukemia Lymphocyte Cultures: Comparison with Whole Blood Cultures and in Situ Hybridization
作者:
BrizardAndre,
BrizardFranchise,
DreyfusBrigitte,
MorelFranck,
ClaudeJean,
TanzerJoseph,
LouisJean,
期刊:
Leukemia&Lymphoma
(Taylor Available online 1993)
卷期:
Volume 11,
issue 5-6
页码: 379-385
ISSN:1042-8194
年代: 1993
DOI:10.3109/10428199309067929
出版商: Taylor&Francis
关键词: B-CLL;Purified B lymphocytes;Karyotype;In situ hybridization
数据来源: Taylor
摘要:
Chromosomal analysis of stimulated whole blood cells and purified B lymphocytes was performed in 13 stage A(0) and 1 stage C(1V) chronic lymphocytic leukemia (B-CLL) patients. Abnormal clones were found in 6 cases in purified B lymphocytes cultures and in a single one in whole blood cultures. In situ hybridization with a chromosome 12 probe was in accordance with the chromosomal analysis of purified B-CLL lymphocytes and not with the results obtained using whole blood culture. Cytogenetic analysis of isolated B cells is simple and sensitive. It enhances the detection of abnormal clones in B-CLL and applied to larger series of patients, it should allow a precise evaluation of the incidence of chromosomal abnormalities in CLL and of their clinical (prognostic) significance.
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