ADSORPTION INTERACTION OF NATIVE AND CHEMICALLY ACETYLATED BETA LACTOGLOBULIN AT THE ALUMINA-WATER INTERFACE
作者:
Anuradha Bhaduri,
K. P. Das,
期刊:
Journal of Dispersion Science and Technology
(Taylor Available online 1994)
卷期:
Volume 15,
issue 2
页码: 165-188
ISSN:0193-2691
年代: 1994
DOI:10.1080/01932699408943551
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Adsorption isotherms of β-lactoglobulin (p-LG) and acetylated β-lactoglobulin (Ac-β-LG). at the alumina/water interface are compared at pH value in the range 3·7 to 9·0.The isotherms of both modified and unmodified β-LG show highly pH-dependent behaviour. Both proteins showed the highest adsorption at pH 5·1. The greatest contrast between β-LG and Ac-β-LG occurred at pH 3·7 and 4·2 where β-LG showed positive adsorption and Ac-β-LG showed highly negative adsorption at the alumina/water interface. Aggregation of proteins leads to higher adsorption at pH Above pH 5·1, adsorption decreased to almost zero for both β-LG and Ac-β-LG, but on further increasing the pH to 9·0, adsorption becomes positive again. These results were related to the aggregation / dissociation property of β-LG in the entire pH range.
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