Preparation and application of polymer‐entrapped enzymes and microorganisms in microbial transformation processes with special reference to steroid 11‐β‐hydroxylation and Δ1‐dehydrogenation
作者:
Klaus Mosbach,
Per‐Olof Larsson,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1970)
卷期:
Volume 12,
issue 1
页码: 19-27
ISSN:0006-3592
年代: 1970
DOI:10.1002/bit.260120103
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractFungal cells fromCurvularia lunatawere entrapped in a crosslinked polyacrylamide gel. The gel‐cells obtained as granules were applied in the microbial transformation of Reichstein compound S leading to cortisol through an 11‐β‐hydroxylation step. Some kinetic studies of this conversion using gel‐cells were carried out. In addition, it was shown that gel‐cell granules which had lost part of their 11‐β‐hydroxylase activity on storage could be reactivated yielding preparations with increased activity.FromCorynebacterium simplexa steroid dehydrogenase catalyzing the Δ1‐ dehydrogenation of cortisol leading to prednisolone was isolated and partially purified. The preparation was entrapped in a crosslinked polyacrylamide gel and the gel‐enzyme granules obtained used in steroid dehyd
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