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CD27/CD70, CD134/CD134 ligand, and CD30/CD153 pathways are independently essential for ...
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CD27/CD70, CD134/CD134 ligand, and CD30/CD153 pathways are independently essential for generation of regulatory cells after intratracheal delivery of alloantigen
作者:
Osamu Aramaki,
Nozomu Shirasugi,
Yoshinobu Akiyama,
Shintaro Shibutani,
Tadatoshi Takayama,
Motohide Shimazu,
Masaki Kitajima,
Yoshifumi Ikeda,
Ko Okumura,
Hideo Yagita,
Masanori Niimi,
期刊:
Transplantation
(OVID Available online 2003)
卷期:
Volume 76,
issue 5
页码: 772-776
ISSN:0041-1337
年代: 2003
出版商: OVID
数据来源: OVID
摘要:
Background.We investigated whether blockade of tumor necrosis factor receptor–ligand pathways could generate regulatory cells induced by intratracheal delivery of alloantigen.Methods.CBA (H-2k) mice were pretreated with intratracheal delivery of splenocytes (1×107) from C57BL/10 (H-2b) mice and intraperitoneal administration of monoclonal antibody (mAb) specific for CD70, CD134 ligand (CD134L), CD153, or CD137L. Seven days later, C57BL/10 hearts were transplanted into pretreated CBA mice. Some naive CBA mice underwent adoptive transfer of splenocytes (5×107) from pretreated CBA mice and transplantation of a C57BL/10 heart on the same day.Results.Untreated CBA mice rejected C57BL/10 cardiac grafts acutely (median survival time [MST] 12 days). Pretreatment with intratracheal delivery of C57BL/10 donor splenocytes prolonged graft survival significantly (MST 84 days). Mice given intratracheal delivery of alloantigen plus anti-CD70, anti-CD134L, or anti-CD153 mAb, but not those given intratracheal delivery of alloantigen plus anti-CD137L mAb, rejected their graft acutely (MST 16, 14, 10, and 65 days, respectively). Adoptive transfer of splenocytes from mice pretreated with intratracheal delivery of alloantigen plus anti-CD70, CD134L, or CD153 mAb did not prolong survival of C57BL/10 cardiac grafts in naive secondary CBA recipients (MST 14, 11, and 11 days, respectively), whereas adoptive transfer of splenocytes from mice given intratracheal delivery of alloantigen plus anti-CD137L mAb did (MST 75 days).Conclusion.The CD27/CD70, CD134/CD134L, and CD30/CD153 pathways are independently required for generation of regulatory cells in our model.
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