A pressure‐tuning FTIR spectroscopic technology for the investigation of the structural and dynamic properties at the molecular level in biological cells and tissues has been developed in our laboratory. This allowed us to study the molecular basis of various biomedical events including structural and dynamic changes of bio‐molecules in diseased tissues and cells. After a brief introduction of this technology and a summary of various biomedical applications of this technology, details of the biomedical applications to the study of structural changes in bio‐molecules of human tissues during the neoplastic transition and to the screening of human cervical cancer and precancerous lesions including clinical statistics are given. © 2004 American Institute of Physics