SUCCESSFUL 48‐HOUR PRESERVATION OF THE RAT LIVER BY CONTINUOUS HYPOTHERMIC PERFUSION WITH HAEMACCEL‐ISOTONIC CITRATE SOLUTION
作者:
T. TAMAKI,
N. KAMADA,
D. WIGHT,
D. PEGG,
期刊:
Transplantation
(OVID Available online 1987)
卷期:
Volume 43,
issue 4
页码: 468-471
ISSN:0041-1337
年代: 1987
出版商: OVID
数据来源: OVID
摘要:
Eleven rat livers were stored at 7°C for 24 or 48 hr using a continuous nonpulsatile perfusion method. The perfusate was based on the isotonic citrate preservation solution but contained, in addition, gelatin polypeptides (Haemaccel, Hoechst) and fluorocarbon emulsion (FC-43, Green Cross Corp.).Isologous livers were orthotopically transplanted after preservation, and long-term survival was 3/6 following 24 hr preservation and 4/5 following 48 hr preservation. All the biopsies taken immediately after re-vascularization were histologically normal. The seven surviving animals were killed at two months and histology showed biliary obstruction, but in all cases the hepatocytes appeared to be well preserved. These late histological findings are common in any use of this transplantation model and are believed to be associated with the difficulty of obtaining a satisfactory anastomosis of the bile duct.The perfusate described here is capable of providing reliable 48 hr preservation of the rat liver.
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