A pH-Stat Approach to the Enzymatic Inhibition Analysis of Some Selected Serum Cholinesterase Inhibitors
作者:
Barrie Tan,
J.Keith Grime,
期刊:
Analytical Letters
(Taylor Available online 1979)
卷期:
Volume 12,
issue 15
页码: 1551-1563
ISSN:0003-2719
年代: 1979
DOI:10.1080/00032717908067934
出版商: Taylor & Francis Group
关键词: Enzyme inhibition;serum cholinesterase;pH-stat analysis
数据来源: Taylor
摘要:
An enzymatic method is described for the pH-stat determination of a series of alkaloids, vitamins, sulfonamides and anions based on the inhibitory effect on the cholinesterase-catalyzed hydrolysis of butyrylcholine iodide (BuCh). All analyses were done at 25.0°C and pH 8.00 Creadable reproducibility of ± 0.02 pH unit)- Sensitivity ranges from 13 ng (physostigmine) to 0.86 g (niacinamide). The precision is < 2.0% with the exception of physostigmine which is ≤ 5.2%. The average recovery and analysis times were (98.1–104.1)% and 10 min., respectively. Complete Inhibition curves are presented and the relative inhibitory “potency” of the compounds examined is inferred.
点击下载:
PDF (365KB)
返 回