Erichrome Stain for Myelin on Osmicated Tissue Embedded in Glycol Methacrylate Plastic
作者:
PierceMabel,
RodriguezMoses,
期刊:
Journal of Histotechnology
(Taylor Available online 1989)
卷期:
Volume 12,
issue 1
页码: 35-36
ISSN:0147-8885
年代: 1989
DOI:10.1179/his.1989.12.1.35
出版商: Taylor&Francis
关键词: Demyelination;myelin;Theiler's virus
数据来源: Taylor
摘要:
AbstractThis paper describes the modification of the Lieb erichrome technique for staining myelin sheaths' embedded in glycol methacrylate plastic. The technique permits excellent morphologic detail of myelinated and demyelinated axons in the central nervous system and the characterization of pathologic abnormalities in the spinal cord of mice persistently infected with a demyelinating strain of Theiler's virus. It is a simple, rapid technique that allows 25–30 spinal cord cross-sections to be embedded, sectioned and stained at one time. (The J Histotechnol12:35, 1989).
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