Improved autoradiographic procedure for cytogenetics: inverted coverslips and double illumination
作者:
M. Stoll,
H. Cardoso,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1981)
卷期:
Volume 31,
issue 2
页码: 101-103
ISSN:1424-8581
年代: 1981
DOI:10.1159/000131631
出版商: S. Karger AG
数据来源: Karger
摘要:
The method described here has the advantage of presenting a clear image of both chromosomes and silver grains. Chromosomes are stained through the emulsion with Hoechst 33258. As two different sources of light are employed—epi-ultraviolet illumination and transmitted visible light—a separate photographic record of each optical plane can be obtained of chromosomes, silver grains, and chromosomes and grains toget
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